BEGIN:VCALENDAR VERSION:2.0 PRODID:-//Eventin//Event Calendar//EN CALSCALE:GREGORIAN METHOD:PUBLISH BEGIN:VEVENT UID:2dd3952d5987124aeda7d3ba92612431@egytraining.org SUMMARY:Heterotrophic Plate Count – Standard Method for the Examination o f Water and Wastewater (9215 A &\; B) DESCRIPTION:Introduction\nIn today’s evolving water and wastewater manage ment sector\, the ability to perform accurate microbiological assessments i s critical for ensuring public health and environmental safety. The Heterot rophic Plate Count (HPC) is a key microbiological test used worldwide to ev aluate the general microbial population in potable water\, treated effluent s\, and other water samples. Standard Method 9215 A & B outlines the essent ial procedures and quality control measures to achieve reliable results in these contexts.\nThis comprehensive training course provides in-depth knowl edge and hands-on experience in applying the HPC using Standard Method 9215 A & B. Participants will learn the principles\, techniques\, and best prac tices that are vital for water quality assessments and compliance with inte rnational standards such as WHO\, EPA\, and local regulatory bodies.\nCours e Objectives\nBy the end of this course\, participants will be able to:\n\n Understand the importance and applications of heterotrophic bacteria testin g in water systems.\nApply Standard Methods 9215 A (Overview) and 9215 B (P our/Spread Plate Methods).\nCorrectly collect\, preserve\, and handle water samples for HPC.\nUse proper media\, incubation\, and enumeration techniqu es for accurate HPC procedures.\nInterpret HPC results and integrate them i nto comprehensive water quality assessments.\n\nCourse Outlines\nDay 1: Mic robial Water Quality and HPC Overview\n\nIntroduction to microbial indicato rs in water quality.\nRole of heterotrophic bacteria in water systems.\nOve rview of HPC and its limitations.\nRegulatory relevance of HPC (WHO\, EPA\, local).\nComparison of different HPC methods.\n\nDay 2: Understanding Stan dard Method 9215 A & B\n\nScope and background of Standard Method 9215.\nDe finitions and microbiological terms.\nOverview of parts A–E of the method .\nFocus on Part A (Introduction) and Part B (Plate Count Methods).\nOvervi ew of pour plate vs spread plate techniques.\n\nDay 3: Laboratory Setup and Media Preparation\n\nRequired materials\, glassware\, and equipment.\nType s of media used (R2A\, PCA\, etc.).\nMedia preparation and sterilization te chniques.\nPour plate media vs spread plate media handling.\nStorage and sh elf-life of prepared media.\n\nDay 4: Sample Collection and Preservation\n\ nSelection of sampling points and volumes.\nAseptic techniques and sample c ontainer types.\nUse of sodium thiosulfate in chlorinated samples.\nSample preservation and holding times.\nSample transport and chain of custody.\n\n Day 5: Pour Plate and Spread Plate Techniques\n\nPour plate procedure – s tep-by-step.\nSpread plate procedure – step-by-step.\nSerial dilution tec hniques.\nAvoiding contamination and false counts.\nDifferences in colony r ecovery between methods.\n\nDay 6: Incubation and Enumeration\n\nIncubation conditions (temperature\, time\, aerobic).\nReading and recording colony c ounts.\nDifferentiating colony morphologies.\nIdentifying overgrowth and TN TC plates.\nCounting accuracy and acceptable plate ranges.\n\nDay 7: Data I nterpretation and Result Reporting\n\nCalculating CFU/mL from dilution fact ors.\nRecording results in lab logs or databases.\nInterpreting normal vs e levated counts.\nLinking results to water quality status.\nReporting format s and compliance with standards.\n\nDay 8: Quality Control and Validation\n \nPositive and negative controls.\nMedia sterility and performance checks.\ nDuplicate and blank sample handling.\nMethod reproducibility and recovery validation.\nTroubleshooting QC failures.\n\nDay 9: Safety\, Waste Disposal \, and Audit Preparedness\n\nBiosafety practices in microbiology labs.\nHan dling biohazard waste and plate disposal.\nCleaning and decontaminating lab surfaces.\nDocumentation for audits and inspections.\nGood Laboratory Prac tice (GLP) compliance.\n\nDay 10: Practical Demonstration and Course Wrap-U p\n\nFull hands-on session using 9215 B (pour and spread plate).\nResult co mparison and discussion.\nReview of key learning points from Days 1–9.\nP articipant assessments and feedback.\nCertification and closing session.\n\ nWhy Attend This Course: Wins & Losses!\n\nGain practical\, actionable skil ls in applying Standard Method 9215 A & B for reliable water quality assess ments.\nStrengthen your ability to detect and monitor heterotrophic bacteri a in potable water and treated effluents.\nBuild confidence in collecting\, preserving\, and analyzing water samples using pour and spread plate techn iques.\nImprove accuracy and compliance in your microbiological testing\, r educing regulatory risks.\nLearn from experienced professionals and real-wo rld examples to reinforce your understanding.\nEnhance your laboratory safe ty practices and ensure audit readiness.\nFoster continuous improvement thr ough quality control and validation practices.\nNetwork with peers and expa nd your knowledge in water microbiology.\n\nConclusion\nAccurate microbial testing is foundational for ensuring the safety and quality of water in tod ay’s dynamic water and wastewater management landscape. This course provi des a structured and comprehensive approach to performing the Heterotrophic Plate Count using Standard Method 9215 A & B\, equipping you with the esse ntial skills\, knowledge\, and confidence to ensure reliable and compliant water quality assessments.\nStart applying these proven methods today and b ecome a leader in water microbiology testing—promoting public health\, en vironmental protection\, and regulatory compliance in your organization. Le t’s build a future where safe water and environmental stewardship are ach ievable goals. LOCATION:Istanbul DTSTAMP:20260611T084649Z DTSTART:20260809T034500Z DTEND:20260822T210500Z END:VEVENT END:VCALENDAR